calcium phosphate precipitation (technique)
- calcium phosphate precipitation (technique)
Кальций-фосфатное осаждение (техника, метод) — метод введения ДНК в клетку без вектора (см. Прямой перенос генов). Основан на осаждении ДНК с помощью кальций-фосфатного комплекса на мембранах клетки. Кальций-фосфатный преципитат образуется после добавления раствора ДНК-CaCl2 в изотонический фосфатный буфер. Такой преципитатный комплекс используется для трансформации бактерий, растительных и животных клеток.
Англо-русский толковый словарь генетических терминов. — М.: Изд-во ВНИРО.
Арефьев В.А., Лисовенко Л.А., науч. ред. Л.И.Патрушев.
1995.
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